Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/16
From 2010.igem.org
(Difference between revisions)
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__NOTOC__ | __NOTOC__ | ||
==2010/10/16 Saturday (Naoto)== | ==2010/10/16 Saturday (Naoto)== | ||
- | '''member''' | + | :'''member''' |
- | naoto and watachin | + | :naoto and watachin |
===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
- | '''material''' | + | :'''material''' |
- | *PCR production (bcsA,bcsB,bcsC and bcsD) | + | :*PCR production (bcsA,bcsB,bcsC and bcsD) |
- | you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html> | + | :you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html> |
- | '''procedure''' | + | :'''procedure''' |
- | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html> |
===Experiment:Digestion=== | ===Experiment:Digestion=== | ||
- | '''material''' | + | :'''material''' |
- | *bcsD | + | :*bcsD |
- | *XbaI | + | :*XbaI |
- | *SpeI | + | :*SpeI |
- | you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html> | + | :you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html> |
- | '''procedure''' | + | :'''procedure''' |
- | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html> |
===Experiment:DNA purification with silica gel=== | ===Experiment:DNA purification with silica gel=== | ||
- | '''material''' | + | :'''material''' |
- | *PCR production(bcsD) | + | :*PCR production(bcsD) |
- | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | + | :you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> |
- | '''procedure''' | + | :'''procedure''' |
- | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> |
===Experiment:Ligation=== | ===Experiment:Ligation=== | ||
- | '''material''' | + | :'''material''' |
- | *bcsD | + | :*bcsD |
- | *pSB1C3 | + | :*pSB1C3 |
- | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html> | + | :you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html> |
- | '''procedure''' | + | :'''procedure''' |
- | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html> | + | :refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html> |
===Experiment:Transformation=== | ===Experiment:Transformation=== | ||
- | '''material''' | + | :'''material''' |
- | *Competent cell(NovaBlue) | + | :*Competent cell(NovaBlue) |
- | *bcsD in pSB1C3 | + | :*bcsD in pSB1C3 |
- | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> | + | :you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> |
- | '''procedure''' | + | :'''procedure''' |
- | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> | + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> |
<br/> | <br/> |
Latest revision as of 17:59, 27 October 2010
![](https://static.igem.org/mediawiki/2010/9/9f/Re-main.png)
E.coli Fiber Project Notebook
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2010/10/16 Saturday (Naoto)
- member
- naoto and watachin
Experiment:Electrophoresis
- material
- PCR production (bcsA,bcsB,bcsC and bcsD)
- you can see other materials at protocol4
- procedure
- see protocol4
Experiment:Digestion
- material
- bcsD
- XbaI
- SpeI
- you can see other materials at protocol6
- procedure
- see protocol6
Experiment:DNA purification with silica gel
- material
- PCR production(bcsD)
- you can see materials at protocol5-2
- procedure
- refer to protocol5-2
Experiment:Ligation
- material
- bcsD
- pSB1C3
- you can see materials at protocol7
- procedure
- refer to protocol7
Experiment:Transformation
- material
- Competent cell(NovaBlue)
- bcsD in pSB1C3
- you can see materials at protocol8
- procedure
- see protocol8