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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/06
From 2010.igem.org
(Difference between revisions)
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==2010/10/06 Wednesday (Naoto)== | ==2010/10/06 Wednesday (Naoto)== | ||
===Experiment:Electrophoresis of insert check PCR=== | ===Experiment:Electrophoresis of insert check PCR=== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto | + | :naoto |
| - | '''material''' | + | :'''material''' |
| - | *insert check PCR production(PCR at 10/05) | + | :*insert check PCR production(PCR at 10/05) |
| - | If you want to know other materials,see protocol4 | + | :If you want to know other materials,see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4] |
| - | '''procedure''' | + | :'''procedure''' |
| - | see protocol4 | + | :see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4] |
| - | '''result''' | + | :'''result''' |
| - | All of bands seemed to be vector self ligation | + | :All of bands seemed to be vector self ligation |
===Experiment:PCR of T7promoter~bcsABCD(''A.xylinum''),T7promoter~bcsEFG(''E.coli''),RBS~T7polymerase=== | ===Experiment:PCR of T7promoter~bcsABCD(''A.xylinum''),T7promoter~bcsEFG(''E.coli''),RBS~T7polymerase=== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto | + | :naoto |
| - | '''material''' | + | :'''material''' |
| - | *A colony of ''A.xylinum'' | + | :*A colony of ''A.xylinum'' |
| - | *bcsEFG(''E.coli'') | + | :*bcsEFG(''E.coli'') |
| - | *T7 polymerase(BBa_K145001) | + | :*T7 polymerase(BBa_K145001) |
| - | If you want to know other materials,see protocol3 | + | :If you want to know other materials,see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3] |
| - | '''procedure''' | + | :'''procedure''' |
| - | see protocol3 | + | :see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3] |
===Experiment:Measure DNA density=== | ===Experiment:Measure DNA density=== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto | + | :naoto |
| - | '''material''' | + | :'''material''' |
| - | *absorption spectrometer | + | :*absorption spectrometer |
| - | *bcsA,B,C,D(''A.xylinum'') | + | :*bcsA,B,C,D(''A.xylinum'') |
| - | *bcsA,B,C,EFG(''E.coli'') | + | :*bcsA,B,C,EFG(''E.coli'') |
| - | *pSB1C3 | + | :*pSB1C3 |
| - | *TE buffer | + | :*TE buffer |
| - | '''procedure''' | + | :'''procedure''' |
| - | *mix 2µl DNA solution with 2μl TE buffer | + | :*mix 2µl DNA solution with 2μl TE buffer |
| - | *apply DNA solution and TE buffer mixture to absorption spectrometer | + | :*apply DNA solution and TE buffer mixture to absorption spectrometer |
| - | *measure DNA density | + | :*measure DNA density |
Revision as of 17:10, 27 October 2010
E.coli Fiber Project Notebook
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2010/10/06 Wednesday (Naoto)
Experiment:Electrophoresis of insert check PCR
- member
- naoto
- material
- insert check PCR production(PCR at 10/05)
- If you want to know other materials,see protocol4
- procedure
- see protocol4
- result
- All of bands seemed to be vector self ligation
Experiment:PCR of T7promoter~bcsABCD(A.xylinum),T7promoter~bcsEFG(E.coli),RBS~T7polymerase
- member
- naoto
- material
- A colony of A.xylinum
- bcsEFG(E.coli)
- T7 polymerase(BBa_K145001)
- If you want to know other materials,see protocol3
- procedure
- see protocol3
Experiment:Measure DNA density
- member
- naoto
- material
- absorption spectrometer
- bcsA,B,C,D(A.xylinum)
- bcsA,B,C,EFG(E.coli)
- pSB1C3
- TE buffer
- procedure
- mix 2µl DNA solution with 2μl TE buffer
- apply DNA solution and TE buffer mixture to absorption spectrometer
- measure DNA density
