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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/25
From 2010.igem.org
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===Experiment:PCR=== | ===Experiment:PCR=== | ||
| - | '''Member'''<br/> | + | :'''Member'''<br/> |
| - | easily | + | :easily |
| - | '''Material'''<br/> | + | :'''Material'''<br/> |
| - | *sense primers of pSB1C3 | + | :templete DNA:pSB1C3 |
| - | *antisense primers of pSB1C3 | + | :*sense primers of pSB1C3 |
| + | :*antisense primers of pSB1C3 | ||
| - | '''Procedure'''<br/> | + | :'''Procedure'''<br/> |
| - | Refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR">protocol3</a></html> | + | :Refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR">protocol3</a></html> |
===Experiment:Ligatiom=== | ===Experiment:Ligatiom=== | ||
Revision as of 14:55, 27 October 2010
E.coli Fiber Project Notebook
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2010/09/25 Saturday(NEX)
Experiment:PCR
- Member
- easily
- Material
- templete DNA:pSB1C3
- sense primers of pSB1C3
- antisense primers of pSB1C3
- Procedure
- Refer to protocol3
Experiment:Ligatiom
Member
easily
Material
- bcsA from E.coli 20μl
- bcsB from E.coli 20μl
- 2×ligation mix 40μl
Procedure
Follow to protocol7
Experiment:Agarose gel electrophoresis
Member
easily
Material
- Today's PCR productions
- Today's ligation productions
- Agarose gel
Procedure
Refer to protocol4
Result
pSB1C3 were appeared
Experiment:DNA purification with silica gel
Member
easily
Materials
- pSB1C3
Procedure
See protocol5-2
Experiment:Ligatiom
Member
easily
Material
- pSB1C3 3μl
- bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
- 2×ligation mix 6μl
Procedure
