"
Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/23
From 2010.igem.org
(Difference between revisions)
(→Experiment:Electrophoresis) |
(→Experiment:Electrophoresis) |
||
| Line 85: | Line 85: | ||
:'''Materials and Procedure''' | :'''Materials and Procedure''' | ||
| - | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis"> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html> |
:'''Result''' | :'''Result''' | ||
Latest revision as of 14:27, 27 October 2010
E.coli Fiber Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010/9/23 Thursday (watachin)
Experiment:PCR
- Member
- naoto
- Materials and Procedure
- E.coli introduced bcsA,B,C,D
- follow protocol3
Experiment:Plating
- Member
- watachin
- Materials and Procedure
- plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
Experiment:Colony PCR
- Member
- naoto
- Materials and Procedure
- Colony PCR production of bcs genes
- follow protocol3
Experiment:Insert check Electrophoresis
- Member
- watachin
- Materials and Procedure
- follow protocol5
- Result
Experiment:Electrophoresis of bcs genes
- Member
- naoto and watachin
- Materials and Procedure
Experiment:DNA Extraction from agarose gel
- Member
- naoto and watachin
- Materials and Procedure
- follow protocol5
Experiment:Electrophoresis
- Member
- naoto and watachin
- Materials and Procedure
- Colony PCR production
- follow protocol4
- Result
Experiment:Transformation
- Member
- watachin
- Materials and Procedure
- follow protocol9
Experiment:Electrophoresis
- Member
- naoto and watachin
- Materials and Procedure
- follow protocol4
- Result
Experiment:DNA Extraction from agarose gel
- Member
- naoto and watachin
- Materials and Procedure
- follow protocol5
