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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/23
From 2010.igem.org
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| - | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR"> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html> |
===Experiment:Plating=== | ===Experiment:Plating=== | ||
Revision as of 14:25, 27 October 2010
E.coli Fiber Project Notebook
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2010/9/23 Thursday (watachin)
Experiment:PCR
- Member
- naoto
- Materials and Procedure
- E.coli introduced bcsA,B,C,D
- follow protocol3
Experiment:Plating
- Member
- watachin
- Materials and Procedure
- plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
Experiment:Insert check PCR
- Member
- naoto
- Materials and Procedure
- Colony PCR production of bcs genes
- follow the protocol(Direct PCR)
Experiment:Insert check Electrophoresis
- Member
- watachin
- Materials and Procedure
- Result
Experiment:Electrophoresis of bcs genes
- Member
- naoto and watachin
- Materials and Procedure
Experiment:DNA Extraction from agarose gel
- Member
- naoto and watachin
- Materials and Procedure
- follow protocol5
Experiment:Electrophoresis
- Member
- naoto and watachin
- Materials and Procedure
- Colony PCR production
- follow the protocol(Electrophoresis)
- Result
Experiment:Transformation
- Member
- watachin
- Materials and Procedure
- follow the protocol(Transformation)
Experiment:Electrophoresis
- Member
- naoto and watachin
- Materials and Procedure
- Result
Experiment:DNA Extraction from agarose gel
- Member
- naoto and watachin
- Materials and Procedure
- follow protocol5
