Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/23

From 2010.igem.org

(Difference between revisions)
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html>
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html>
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===Experiment:Plating===
===Experiment:Plating===
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plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
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===Experiment:Insert check PCR===
===Experiment:Insert check PCR===
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'''Result'''
'''Result'''
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===Experiment:DNA purification from agarose gel with QIAGEN===
===Experiment:DNA purification from agarose gel with QIAGEN===
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'''Materials and Procedure'''
'''Materials and Procedure'''
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
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===Experiment:Insert check Electrophoresis===
===Experiment:Insert check Electrophoresis===
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'''Materials and Procedure'''
'''Materials and Procedure'''
-
 
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html>
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html>
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===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
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'''Materials and Procedure'''
'''Materials and Procedure'''
-
 
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>

Revision as of 14:19, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/9/23 Thursday (watachin)

Experiment:PCR

Member
naoto

Materials and Procedure

Direct PCR of bcs genes

follow the protocol(Direct PCR)

Experiment:Plating

Member
watachin

Materials and Procedure

plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth

Experiment:Insert check PCR

Member
naoto

Materials and Procedure

Insert check of bcs genes

follow the protocol(Direct PCR)

Experiment:Insert check Electrophoresis

Member
watachin

Materials and Procedure

follow the protocol(Electrophoresis)

Result

2010-09-23-01.jpg 2010-09-23-02.jpg

Experiment:Electrophoresis of bcs genes

Member
naoto and watachin

Materials and Procedure

follow the protocol(Electrophoresis)

Result

Experiment:DNA purification from agarose gel with QIAGEN

Member
naoto and watachin

Materials and Procedure

follow the protocol(DNA purification from agarose gel with QIAGEN)

Experiment:Insert check Electrophoresis

Member
naoto and watachin

Materials and Procedure

follow the protocol(Electrophoresis)

Result

2010-09-23-03.jpg

Experiment:Transformation

Member
watachin

Materials and Procedure

follow the protocol(Transformation)

Experiment:Electrophoresis

Member
naoto and watachin

Materials and Procedure

follow the protocol(Electrophoresis)

Result

2010-09-23-04.jpg

Experiment:DNA purification from agarose gel with QIAGEN

Member
naoto and watachin

Materials and Procedure

follow the protocol(DNA purification from agarose gel with QIAGEN)