Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/22

From 2010.igem.org

(Difference between revisions)
(→Experiment:PCR bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3)
(→Experiment:Electrophoresis of bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3)
Line 37: Line 37:
:'''Material'''
:'''Material'''
-
:*PCR production (bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C)
+
:*PCR production (bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C)
:If you want to know other materials,refer to protocol4
:If you want to know other materials,refer to protocol4
:'''Procedure'''
:'''Procedure'''
-
:see you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol4</a></html>
+
:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol4</a></html>
:'''Result'''
:'''Result'''

Revision as of 14:15, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/09/22 Wednesday (Naoto)

Experiment:PCR bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

Member
naoto and NEX
Material
  • bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3
you can see other materials at protocol3
Procedure
protocol3

Experiment:Colony PCR

Member
naoto and NEX
Material
  • bcsA,B in E.coli
you can see other materials at protocol3
Procedure
you can see other materials at protocol3

Experiment:Electrophoresis of bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

Member
naoto and NEX
Material
  • PCR production (bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C)
If you want to know other materials,refer to protocol4
Procedure
see protocol4
Result
Bands of bcsB(A.xylinum) and bcsA,B,C(E.coli) could be seen

Experiment:Colony PCR

Member
naoto and NEX
Material
  • PCR production (bcsA,B)
you can see other materials at you can see other materials at protocol3
Procedure
you can see other materials at protocol3
Result
a band of No.41(bcsB,Axylinum) appeared slightly

Experiment:Purification of bcsB(A.xylinum) and bcsA,B,C(E.coli) with QIAGEN

Member
naoto and NEX
Material
  • bcsB(A.xylinum) and bcsA,B,C(E.coli) in agarose gel
you can see other materials at protocol5
Procedure
see protocol5

Experiment:digestion of bcsB(A.xylinum) and bcsA,B,C(E.coli)

Member
naoto and NEX
Material
  • bcsB(A.xylinum) and bcsA,B,C(E.coli)
you can see other materials at you can see other materials at protocol7


Procedure
see you can see other materials at protocol7


Preparation:Make preculture

Member
naoto and NEX
Material
  • LB+Cam　Broth
  • a colony of E.coli having bcsB(A.xylinum)
you can see other materials at you can see other materials at protocol2


Procedure
see you can see other materials at protocol2
Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/22"