Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/22

From 2010.igem.org

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==2010/09/22 Wednesday (Naoto)==
==2010/09/22 Wednesday (Naoto)==
===Experiment:PCR bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3===
===Experiment:PCR bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3===
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:naoto and NEX
:naoto and NEX
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:'''Material'''
:*bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3
:*bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html>
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html>
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:<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#">protocol3</a></html>
:<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#">protocol3</a></html>

Revision as of 14:13, 27 October 2010


E.coli Fiber Project Notebook

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2010/09/22 Wednesday (Naoto)

Experiment:PCR bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

Member
naoto and NEX
Material
  • bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3
you can see other materials at protocol3
Procedure
protocol3

Experiment:Colony PCR

Member
naoto and NEX
Material
  • bcsA,B in E.coli
you can see other materials at protocol3
Procedure
you can see other materials at protocol3

Experiment:Electrophoresis of bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

Member
naoto and NEX
Material
  • PCR production (bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C)
If you want to know other materials,refer to protocol4
Procedure
see you can see other materials at protocol4
Result
Bands of bcsB(A.xylinum) and bcsA,B,C(E.coli) could be seen

Experiment:Colony PCR

Member
naoto and NEX
Material
  • PCR production (bcsA,B)
you can see other materials at you can see other materials at protocol3
Procedure
you can see other materials at protocol3
Result
a band of No.41(bcsB,Axylinum) appeared slightly

Experiment:Purification of bcsB(A.xylinum) and bcsA,B,C(E.coli) with QIAGEN

Member
naoto and NEX
Material
  • bcsB(A.xylinum) and bcsA,B,C(E.coli) in agarose gel
you can see other materials at protocol5
Procedure
see protocol5

Experiment:digestion of bcsB(A.xylinum) and bcsA,B,C(E.coli)

Member
naoto and NEX
Material
  • bcsB(A.xylinum) and bcsA,B,C(E.coli)
you can see other materials at you can see other materials at protocol7


Procedure
see you can see other materials at protocol7


Preparation:Make preculture

Member
naoto and NEX
Material
  • LB+Cam　Broth
  • a colony of E.coli having bcsB(A.xylinum)
you can see other materials at you can see other materials at protocol2


Procedure
see you can see other materials at protocol2
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