Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/22

From 2010.igem.org

(Difference between revisions)
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==2010/09/22 Wednesday (Naoto)==
==2010/09/22 Wednesday (Naoto)==
===Experiment:PCR bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3===
===Experiment:PCR bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3===
-
'''member'''
+
:'''member'''
-
naoto and NEX
+
:naoto and NEX
-
'''material'''
+
:'''material'''
-
*bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3
+
:*bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3
-
you can see other materials at protocol3
+
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html>
-
'''procedure'''
+
:'''procedure'''
-
protocol3
+
:<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#">protocol3</a></html>
-
===Experiment:Insert　check PCR===
+
===Experiment:Colony PCR===
-
'''member'''
+
:'''member'''
-
naoto and NEX
+
:naoto and NEX
-
'''material'''
+
:'''material'''
-
*Colonies of E.coli having bcsA,B(''A.xylinum'')
+
:*bcsA,B in E.coli
-
you can see other materials at protocol3
+
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html>
-
'''procedure'''
+
:'''procedure'''
-
protocol3
+
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html>
===Experiment:Electrophoresis of bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3===
===Experiment:Electrophoresis of bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C3===
-
'''member'''
+
:'''Member'''
-
naoto and NEX
+
:naoto and NEX
-
'''material'''
+
:'''Material'''
-
*PCR production (bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C)
+
:*PCR production (bcsABCD,bcsB(''A.xylinum''),bcsA,B,C(''E.coli'') and pSB1C)
-
If you want to know other materials,refer to protocol4
+
:If you want to know other materials,refer to protocol4
-
'''procedure'''
+
:'''Procedure'''
-
see protocol4
+
:see you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol4</a></html>
-
'''result'''
+
:'''Result'''
-
Bands of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') could be seen
+
:Bands of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') could be seen
-
===Experiment:miniprep parts of bcsB,C(''E.coli'')===
+
===Experiment:Colony PCR===
-
'''member'''
+
:'''Member'''
-
naoto and NEX
+
:naoto and NEX
-
'''material'''
+
:'''Material'''
-
*bcsB,C(''E.coli'') in pSB1C3
+
:*PCR production (bcsA,B)
-
If you want to know other materials,refer to protocol9
+
:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html>
-
'''procedure'''
+
:'''Procedure'''
-
see protocol9
+
:you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol3</a></html>
-
===Experiment:Insert　check PCR===
+
:'''Result'''
-
'''member'''
+
-
naoto and NEX
+
:a band of No.41(bcsB,''Axylinum'') appeared slightly
-
'''material'''
+
===Experiment:Purification of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') with QIAGEN===
 +
:'''Member'''
-
*Insert check PCR production (bcsA,B(''A.xylinum''))
+
:naoto and NEX
-
you can see other materials at protocol3
+
-
'''procedure'''
+
:'''Material'''
-
protocol3
+
:*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') in agarose gel
 +
:you can see other materials at protocol5
-
'''result'''
+
:'''Procedure'''
-
a band of No.41(bcsB,''Axylinum'') appeared slightly
+
:see protocol5
-
===Experiment:Purification of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') with QIAGEN===
+
===Experiment:digestion of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')===
-
'''member'''
+
:'''Member'''
-
naoto and NEX
+
:naoto and NEX
-
'''material'''
+
:'''Material'''
 +
:*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')
 +
:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol7</a></html>
-
*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'') in agarose gel
 
-
you can see other materials at protocol5
 
-
 
-
'''procedure'''
 
-
 
-
see protocol5
 
-
 
-
===Experiment:digestion of bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')===
 
-
'''member'''
 
-
naoto and NEX
+
:'''Procedure'''
-
'''material'''
+
:see you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol7</a></html>
-
*bcsB(''A.xylinum'') and bcsA,B,C(''E.coli'')
+
-
you can see other materials at protocol6
+
-
'''procedure'''
 
-
see protocol6
+
===Preparation:Make preculture===
 +
:'''Member'''
-
===Experiment:Subculture ''E.coli'' having bcsB(''A.xylinum'')===
+
:naoto and NEX
-
'''member'''
+
-
naoto and NEX
+
:'''Material'''
-
'''material'''
+
:*LB+Cam　Broth
 +
:*a colony of E.coli having bcsB(''A.xylinum'')
 +
:you can see other materials at you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol2</a></html>
-
*LB+Cam　Broth
 
-
*a colony of E.coli having bcsB(''A.xylinum'')
 
-
you can see other materials at protocol1
 
-
'''procedure'''
+
:'''Procedure'''
-
see protocol1
+
:see you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol">protocol2</a></html>

Revision as of 14:12, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/09/22 Wednesday (Naoto)

Experiment:PCR bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

member
naoto and NEX
material
  • bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3
you can see other materials at protocol3
procedure
protocol3

Experiment:Colony PCR

member
naoto and NEX
material
  • bcsA,B in E.coli
you can see other materials at protocol3
procedure
you can see other materials at protocol3

Experiment:Electrophoresis of bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

Member
naoto and NEX
Material
  • PCR production (bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C)
If you want to know other materials,refer to protocol4
Procedure
see you can see other materials at protocol4
Result
Bands of bcsB(A.xylinum) and bcsA,B,C(E.coli) could be seen

Experiment:Colony PCR

Member
naoto and NEX
Material
  • PCR production (bcsA,B)
you can see other materials at you can see other materials at protocol3
Procedure
you can see other materials at protocol3
Result
a band of No.41(bcsB,Axylinum) appeared slightly

Experiment:Purification of bcsB(A.xylinum) and bcsA,B,C(E.coli) with QIAGEN

Member
naoto and NEX
Material
  • bcsB(A.xylinum) and bcsA,B,C(E.coli) in agarose gel
you can see other materials at protocol5
Procedure
see protocol5

Experiment:digestion of bcsB(A.xylinum) and bcsA,B,C(E.coli)

Member
naoto and NEX
Material
  • bcsB(A.xylinum) and bcsA,B,C(E.coli)
you can see other materials at you can see other materials at protocol7


Procedure
see you can see other materials at protocol7


Preparation:Make preculture

Member
naoto and NEX
Material
  • LB+Cam　Broth
  • a colony of E.coli having bcsB(A.xylinum)
you can see other materials at you can see other materials at protocol2


Procedure
see you can see other materials at protocol2
Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/22"