Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/21

From 2010.igem.org

(Difference between revisions)
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==2010/9/21 Tuesday (watachin)==
==2010/9/21 Tuesday (watachin)==
===Experiment:Miniprep (extraction of plasmid kit)===
===Experiment:Miniprep (extraction of plasmid kit)===
-
'''Member'''<br />
+
:'''Member'''<br />
-
easily and NEX
+
:easily and NEX
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Miniprep_.28extraction_of_plasmid_kit.29">protocol10</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Miniprep_.28extraction_of_plasmid_kit.29">protocol10</a></html>
===Experiment:PCR===
===Experiment:PCR===
-
'''Member'''<br />
+
:'''Member'''<br />
-
watachin
+
:watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
PCR of pSB1C3
+
:PCR of pSB1C3
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
'''Member'''<br />
'''Member'''<br />
-
easily
+
:easily
'''Materials and Procedure'''
'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
'''Result'''
'''Result'''
Line 35: Line 35:
===Experiment:DNA purification from agarose gel===
===Experiment:DNA purification from agarose gel===
'''Member'''<br />
'''Member'''<br />
-
watachin
+
:watachin
'''Materials and Procedure'''
'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>
===Experiment:Digestion===
===Experiment:Digestion===
'''Member'''<br />
'''Member'''<br />
-
NEX and watachin
+
:NEX and watachin
'''Materials and Procedure'''
'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">protocol7</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">protocol7</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
'''Member'''<br />
'''Member'''<br />
-
NEX and watachin
+
:NEX and watachin
'''Materials and Procedure'''
'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol5</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol5</a></html>
'''Result'''
'''Result'''
Line 63: Line 63:
===Experiment:DNA purification from agarose gel===
===Experiment:DNA purification from agarose gel===
'''Member'''<br />
'''Member'''<br />
-
NEX and watachin
+
:NEX and watachin
'''Materials and Procedure'''
'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
'''Member'''<br />
'''Member'''<br />
-
watachin
+
:watachin
'''Materials and Procedure'''
'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
'''Result'''
'''Result'''
Line 84: Line 84:
===Experiment:DNA purification from agarose gel with QIAGEN===
===Experiment:DNA purification from agarose gel with QIAGEN===
'''Member'''<br />
'''Member'''<br />
-
watachin
+
:watachin
'''Materials and Procedure'''
'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
===Experiment:Transformation===
===Experiment:Transformation===
'''Member'''<br />
'''Member'''<br />
-
watachin
+
:watachin
'''Materials and Procedure'''
'''Materials and Procedure'''
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html>

Revision as of 13:45, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/9/21 Tuesday (watachin)

Experiment:Miniprep (extraction of plasmid kit)

Member
easily and NEX
Materials and Procedure
follow protocol10

Experiment:PCR

Member
watachin
Materials and Procedure
PCR of pSB1C3
follow protocol3

Experiment:Electrophoresis

Member

easily

Materials and Procedure

follow protocol4

Result

2010-09-21-01.jpg 2010-09-21-02.jpg

Experiment:DNA purification from agarose gel

Member

watachin

Materials and Procedure

follow protocol5

Experiment:Digestion

Member

NEX and watachin

Materials and Procedure

follow protocol7

Experiment:Electrophoresis

Member

NEX and watachin

Materials and Procedure

follow protocol5

Result

2010-09-21-03.jpg

Experiment:DNA purification from agarose gel

Member

NEX and watachin

Materials and Procedure

follow protocol5

Experiment:Electrophoresis

Member

watachin

Materials and Procedure

follow the protocol(Electrophoresis)

Result

2010-09-21-04.jpg 2010-09-21-05.jpg

Experiment:DNA purification from agarose gel with QIAGEN

Member

watachin

Materials and Procedure

follow the protocol(DNA purification from agarose gel with QIAGEN)

Experiment:Transformation

Member

watachin

Materials and Procedure

follow the protocol(Transformation)
Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/21"