Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/21

From 2010.igem.org

(Difference between revisions)

Revision as of 13:43, 27 October 2010

E.coli Fiber Project Notebook

2010/9/21 Tuesday (watachin)

Experiment:Miniprep (extraction of plasmid kit)

Member
easily and NEX

Materials and Procedure

follow protocol10

Experiment:PCR

Member
watachin

Materials and Procedure

PCR of pSB1C3

follow protocol3

Experiment:Electrophoresis

Member
easily

Materials and Procedure

follow protocol4

Result

Experiment:DNA purification from agarose gel

Member
watachin

Materials and Procedure

follow protocol5

Experiment:Digestion

Member
NEX and watachin

Materials and Procedure

follow protocol7

Experiment:Electrophoresis

Member
NEX and watachin

Materials and Procedure

follow protocol5

Result

Experiment:DNA purification from agarose gel

Member
NEX and watachin

Materials and Procedure

follow protocol5

Experiment:Electrophoresis

Member
watachin

Materials and Procedure

follow the protocol(Electrophoresis)

Result

Experiment:DNA purification from agarose gel with QIAGEN

Member
watachin

Materials and Procedure

follow the protocol(DNA purification from agarose gel with QIAGEN)

Experiment:Transformation

Member
watachin

Materials and Procedure

follow the protocol(Transformation)

@@ Line 8: / Line 8: @@
 '''Materials and Procedure'''
-follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Miniprep_.28extraction_of_plasmid_kit.29">the protocol(Miniprep)</a></html>
+follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Miniprep_.28extraction_of_plasmid_kit.29">protocol10</a></html>
 ===Experiment:PCR===
@@ Line 19: / Line 18: @@
 PCR of pSB1C3
-follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html>
+follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
 ===Experiment:Electrophoresis===
@@ Line 29: / Line 26: @@
 '''Materials and Procedure'''
-follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
 '''Result'''
@@ Line 36: / Line 33: @@
 [[Image:2010-09-21-02.jpg]]
-===Experiment:DNA purification from agarose gel with QIAGEN===
+===Experiment:DNA purification from agarose gel===
 '''Member'''<br />
 watachin
@@ Line 42: / Line 39: @@
 '''Materials and Procedure'''
+follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>
-follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
 ===Experiment:Digestion===
@@ Line 52: / Line 47: @@
 '''Materials and Procedure'''
-follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">the protocol(Digestion)</a></html>
+follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">protocol7</a></html>
-===Experiment:Digestion===
-'''Member'''<br />
-NEX and watachin
-'''Materials and Procedure'''
-follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">the protocol(Digestion)</a></html>
 ===Experiment:Electrophoresis===
@@ Line 69: / Line 55: @@
 '''Materials and Procedure'''
+follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol5</a></html>
-follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
 '''Result'''
@@ Line 77: / Line 61: @@
 [[Image:2010-09-21-03.jpg]]
+===Experiment:DNA purification from agarose gel===
-===Experiment:DNA purification from agarose gel with QIAGEN===
 '''Member'''<br />
 NEX and watachin
@@ Line 84: / Line 67: @@
 '''Materials and Procedure'''
+follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>
-follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
 ===Experiment:Electrophoresis===
@@ Line 92: / Line 74: @@
 '''Materials and Procedure'''
 follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
 '''Result'''
@@ Line 101: / Line 81: @@
 [[Image:2010-09-21-04.jpg]]
 [[Image:2010-09-21-05.jpg]]
 ===Experiment:DNA purification from agarose gel with QIAGEN===
@@ Line 108: / Line 87: @@
 '''Materials and Procedure'''
 follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
@@ Line 117: / Line 95: @@
 '''Materials and Procedure'''
 follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html>