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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/09
From 2010.igem.org
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==2010/09/09 Thursday (Naoto)== | ==2010/09/09 Thursday (Naoto)== | ||
===Experiment:Ligation=== | ===Experiment:Ligation=== | ||
| - | :''' | + | :'''Member''' |
:naoto and NEX | :naoto and NEX | ||
| - | :''' | + | :'''Material''' |
:*bcsA,B 10µl each | :*bcsA,B 10µl each | ||
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:*ligation Covinience Kit 20µl each | :*ligation Covinience Kit 20µl each | ||
| - | :''' | + | :'''Procedure''' |
:#Add material to PCR tube | :#Add material to PCR tube | ||
:#Incubate (20℃ 10min) | :#Incubate (20℃ 10min) | ||
Latest revision as of 06:41, 27 October 2010
E.coli Fiber Project Notebook
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2010/09/09 Thursday (Naoto)
Experiment:Ligation
- Member
- naoto and NEX
- Material
- bcsA,B 10µl each
- bcsC,D 10µl each
- bcsA,B 10µl each
- ligation Covinience Kit 20µl each
- Procedure
- Add material to PCR tube
- Incubate (20℃ 10min)
Experiment:Electrophoresis
- Member
- naoto and NEX
- Material
- ligation products(see above)
- we show other material at protocol4
- Procedure
- see protocol4
