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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/09
From 2010.igem.org
(Difference between revisions)
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==2010/09/09 Thursday (Naoto)== | ==2010/09/09 Thursday (Naoto)== | ||
===Experiment:Ligation=== | ===Experiment:Ligation=== | ||
| - | '''member''' | + | :'''member''' |
| - | naoto and NEX | + | :naoto and NEX |
| - | '''material''' | + | :'''material''' |
| - | *bcsA,B | + | :*bcsA,B 10µl each |
| - | *bcsC,D | + | :*bcsC,D 10µl each |
| - | *bcsA,B | + | :*bcsA,B 10µl each |
| - | * | + | :*ligation Covinience Kit 20µl each |
| - | '''procedure''' | + | :'''procedure''' |
| - | # | + | :#Add material to PCR tube |
| - | # | + | :#Incubate (20℃ 10min) |
===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
| - | ''' | + | :'''Member''' |
| - | naoto and NEX | + | :naoto and NEX |
| - | ''' | + | :'''Material''' |
| - | *ligation products(see above) | + | :*ligation products(see above) |
| - | we show other material at | + | :we show other material at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| + | :'''Procedure''' | ||
| + | :see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | ||
<br/> | <br/> | ||
Revision as of 06:40, 27 October 2010
E.coli Fiber Project Notebook
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2010/09/09 Thursday (Naoto)
Experiment:Ligation
- member
- naoto and NEX
- material
- bcsA,B 10µl each
- bcsC,D 10µl each
- bcsA,B 10µl each
- ligation Covinience Kit 20µl each
- procedure
- Add material to PCR tube
- Incubate (20℃ 10min)
Experiment:Electrophoresis
- Member
- naoto and NEX
- Material
- ligation products(see above)
- we show other material at protocol4
- Procedure
- see protocol4
