Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/17

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
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==2010/10/17 Sunday (Naoto)==
 +
'''member'''
 +
naoto and watachin
 +
 +
 +
===Experiment:Digestion===
 +
'''material'''
 +
*bcsD
 +
*XbaI
 +
*SpeI
 +
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
 +
 +
'''procedure'''
 +
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html>
 +
 +
===Experiment:DNA purification with silica gel===
 +
'''material'''
 +
*PCR production(bcsD)
 +
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
 +
 +
'''procedure'''
 +
 +
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html>
 +
 +
===Experiment:Ligation===
 +
'''material'''
 +
*bcsD
 +
*pSB1C3
 +
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
 +
 +
'''procedure'''
 +
 +
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
 +
 +
===Experiment:Transformation===
 +
'''material'''
 +
*Competent cell(NovaBlue)
 +
*bcsD in pSB1C3
 +
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
 +
 +
'''procedure'''
 +
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>

Revision as of 17:43, 26 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
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October 2010
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31

2010/10/17 Sunday (Naoto)

member naoto and watachin


Experiment:Digestion

material

  • bcsD
  • XbaI
  • SpeI

you can see other materials at protocol6

procedure see protocol6

Experiment:DNA purification with silica gel

material

  • PCR production(bcsD)

you can see materials at protocol5-2

procedure

refer to protocol5-2

Experiment:Ligation

material

  • bcsD
  • pSB1C3

you can see materials at protocol7

procedure

refer to protocol7

Experiment:Transformation

material

  • Competent cell(NovaBlue)
  • bcsD in pSB1C3

you can see materials at protocol8

procedure

see protocol8
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