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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/14
From 2010.igem.org
(Difference between revisions)
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==2010/10/14 Thursday (Naoto)== | ==2010/10/14 Thursday (Naoto)== | ||
'''member''' | '''member''' | ||
| - | maki and watachin | + | |
| + | naoto,maki and watachin | ||
===Preparation:Measure DNA concentration=== | ===Preparation:Measure DNA concentration=== | ||
| Line 28: | Line 29: | ||
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html> | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html> | ||
| - | < | + | ===Experiment:Electrophoresis=== |
| + | '''material''' | ||
| + | *bcsA | ||
| + | *bcsB | ||
| + | *bcsC | ||
| + | *bcsD | ||
| + | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | ||
| + | |||
| + | '''procedure''' | ||
| + | |||
| + | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | ||
Revision as of 17:06, 26 October 2010
E.coli Fiber Project Notebook
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Contents |
2010/10/14 Thursday (Naoto)
member
naoto,maki and watachin
Preparation:Measure DNA concentration
Material
- bcsA
- bcsB
- bcsC
- bcsD
- pSB1C3
- TE buffer
Procedure
- mix 4μl of DNA and 4μl of TE buffer
- set mixture in absorption spectrometer
- measure DNA concentrations
Experiment:PCR
material
- templete DNA (from A.xylinum and pSB1C3)
you can see other materials at protocol3
procedure
see protocol3
Experiment:Electrophoresis
material
- bcsA
- bcsB
- bcsC
- bcsD
you can see materials at protocol4
procedure
refer to protocol4
