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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/25
From 2010.igem.org
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'''Procedure'''<br/> | '''Procedure'''<br/> | ||
Refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR">protocol3</a></html> | Refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR">protocol3</a></html> | ||
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===Experiment:Ligatiom=== | ===Experiment:Ligatiom=== | ||
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'''Procedure'''<br/> | '''Procedure'''<br/> | ||
Follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol7:Ligation">protocol7</a></html> | Follow to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol7:Ligation">protocol7</a></html> | ||
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===Experiment:Agarose gel electrophoresis=== | ===Experiment:Agarose gel electrophoresis=== | ||
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'''Procedure'''<br /> | '''Procedure'''<br /> | ||
See <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol5-2:DNA_Purification_with_silica_gel">protocol5-2</a></html> | See <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol5-2:DNA_Purification_with_silica_gel">protocol5-2</a></html> | ||
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===Experiment:Ligatiom=== | ===Experiment:Ligatiom=== | ||
Revision as of 16:08, 26 October 2010
E.coli Fiber Project Notebook
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2010/09/25 Saturday(NEX)
Experiment:PCR
Member
easily
Material
- sense primers of pSB1C3
- antisense primers of pSB1C3
Procedure
Refer to protocol3
Experiment:Ligatiom
Member
easily
Material
- bcsA from E.coli 20μl
- bcsB from E.coli 20μl
- 2×ligation mix 40μl
Procedure
Follow to protocol7
Experiment:Agarose gel electrophoresis
Member
easily
Material
- Today's PCR productions
- Today's ligation productions
- Agarose gel
Procedure
Refer to protocol4
Result
pSB1C3 were appeared
Experiment:DNA purification with silica gel
Member
easily
Materials
- pSB1C3
Procedure
See protocol5-2
Experiment:Ligatiom
Member
easily
Material
- pSB1C3 3μl
- bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
- 2×ligation mix 6μl
Procedure
