Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/25

From 2010.igem.org

(Difference between revisions)
(Experiment:Agarose gel electrophoresis)
Line 1: Line 1:
-
==2010/09/10 Saturday(NEX)==
+
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
 +
__NOTOC__
 +
==2010/09/25 Saturday(NEX)==
===Experiment:PCR===
===Experiment:PCR===

Revision as of 16:07, 26 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/09/25 Saturday(NEX)

Experiment:PCR

Member
easily

Material

  • sense primers of pSB1C3
  • antisense primers of pSB1C3

Procedure
Refer to protocol3


Experiment:Ligatiom

Member
easily

Material

  • bcsA from E.coli 20μl
  • bcsB from E.coli 20μl
  • 2×ligation mix 40μl

Procedure
Follow to protocol7


Experiment:Agarose gel electrophoresis

Member
easily

Material

  • Today's PCR productions
  • Today's ligation productions
  • Agarose gel

Procedure
Refer to protocol4

Result
100925.jpg
pSB1C3 were appeared

Experiment:DNA purification with silica gel

Member
easily

Materials

  • pSB1C3

Procedure
See protocol5-2


Experiment:Ligatiom

Member
easily

Material

  • pSB1C3 3μl
  • bcsA,B,C,D from A.xylinum or bcsA from E.coli 3μl
  • 2×ligation mix 6μl

Procedure

Follow to protocol7