Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/14

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==2010/10/14 Thursday (Naoto)==
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'''member'''
 +
maki and watachin
 +
 +
===Preparation:Measure DNA concentration===
 +
'''Material'''
 +
*bcsA
 +
*bcsB
 +
*bcsC
 +
*bcsD
 +
*pSB1C3
 +
*TE buffer
 +
 +
'''Procedure'''
 +
#mix 4μl of DNA and 4μl of TE buffer
 +
#set mixture in absorption spectrometer
 +
#measure DNA concentrations
 +
 +
===Experiment:PCR===
 +
'''material'''
 +
*templete DNA (from A.xylinum and pSB1C3)
 +
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>
 +
 +
'''procedure'''
 +
 +
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol3</a></html>

Revision as of 15:55, 26 October 2010


E.coli Fiber Project Notebook

August 2010
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2010/10/14 Thursday (Naoto)

member maki and watachin

Preparation:Measure DNA concentration

Material

  • bcsA
  • bcsB
  • bcsC
  • bcsD
  • pSB1C3
  • TE buffer

Procedure

  1. mix 4μl of DNA and 4μl of TE buffer
  2. set mixture in absorption spectrometer
  3. measure DNA concentrations

Experiment:PCR

material

  • templete DNA (from A.xylinum and pSB1C3)

you can see other materials at protocol3

procedure

see protocol3
Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/10/14"