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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/10
From 2010.igem.org
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}} | {{:Team:Tokyo_Metropolitan/Notebook/Fiber}} | ||
| - | + | ==2010/10/10 Sunday (Naoto)== | |
'''member''' | '''member''' | ||
| Line 8: | Line 8: | ||
===Preparation:Measure DNA concentration=== | ===Preparation:Measure DNA concentration=== | ||
'''material''' | '''material''' | ||
| + | |||
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | ||
'''procedure''' | '''procedure''' | ||
| + | |||
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | ||
| + | |||
| + | ===Experiment:Ligation=== | ||
| + | '''material'' | ||
| + | |||
| + | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol8(Ligation)</a></html> | ||
| + | |||
| + | '''procedure''' | ||
| + | |||
| + | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> | ||
| + | |||
| + | ===Experiment:Transformation=== | ||
| + | '''material''' | ||
| + | |||
| + | *Competent Cell (Novablue) | ||
| + | *bcsA in pSB1C3 | ||
| + | *bcsB in pSB1C3 | ||
| + | *bcsC in pSB1C3 | ||
| + | *bcsD in pSB1C3 | ||
| + | you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol8</a></html> | ||
| + | |||
| + | '''procedure''' | ||
| + | |||
| + | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol8</a></html> | ||
Revision as of 05:19, 26 October 2010
E.coli Fiber Project Notebook
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Contents |
2010/10/10 Sunday (Naoto)
member
watachin
Preparation:Measure DNA concentration
material
you can see materials at protocol5-2
procedure
refer to protocol5-2
Experiment:Ligation
'material
you can see materials at protocol8(Ligation)
procedure
refer to protocol8
Experiment:Transformation
material
- Competent Cell (Novablue)
- bcsA in pSB1C3
- bcsB in pSB1C3
- bcsC in pSB1C3
- bcsD in pSB1C3
you can see other materials at protocol8
procedure
refer to protocol8
