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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16
From 2010.igem.org
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'''Materials,Procedure''' | '''Materials,Procedure''' | ||
| - | Same | + | |
| + | Same <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html> | ||
PCR Product of insert is pBS1C3 | PCR Product of insert is pBS1C3 | ||
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| - | ==Experiment:Ligation== | + | ===Experiment:Ligation=== |
'''member''' | '''member''' | ||
Revision as of 17:15, 24 October 2010
E.coli Fiber Project Notebook
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Contents |
2010/09/16 Thuesday (Bambi75)
Experiment:PCR
member
naoto and bambi75
materials,Procedure
Same protocol4
- 95℃3min
- 96℃1min
- 51℃1min
- 72℃3min
- 10℃∞
（96℃～72℃/30cycle）
Experiment:Agarose Gels Electro-phoresis
member
Same above
Materials,Procedure
Same protocol4 PCR Product of insert is pBS1C3
Experiment:Experiment:Make Agarose Gels Electro-phoresis
Member
Same above
Materials and Procedure
Same protocol4
Preparation:Medium making
member
Same above
materials and procedure
Same protocol1
Experiment:Ligation
member
Same above
materials and procedure follow protocol8
- bcsA,B and C(E.coli).
- bcsA,B,C and D(A.xylium)
- pSB1C3
