Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/02

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
-
==2010/10/02 Saturday (watachin)==
+
==Electrophoresis==
-
===Electrophoresis===
+
'''member'''
'''member'''
Line 21: Line 20:
[[Image:2010-10-02-02.jpg]]
[[Image:2010-10-02-02.jpg]]
-
===Ligation===
+
==Ligation==
'''member'''
'''member'''
Line 35: Line 34:
You can see our way of ligation at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol8#.E3.80.80">protocol8</a></html>
You can see our way of ligation at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol8#.E3.80.80">protocol8</a></html>
-
===Transformation===
+
==Transformation==
'''member'''
'''member'''

Revision as of 16:14, 24 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

Electrophoresis

member

naoto and easily

material

  • pSB1C3
  • bcsA
  • bcsB
  • bcsC
  • bcsD

all of them had cut with XbaI and SpeI

Result

2010-10-02-01.jpg

2010-10-02-02.jpg

Ligation

member

easily

material

  • pSB1C3
  • bcsC

You can see other materials at protocol8

procedure

You can see our way of ligation at protocol8

Transformation

member

easily

material

  • bcsC in pSB1C3

You can see other materials at protocol9

procedure

You can see our way of ligation at protocol9