Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/03

From 2010.igem.org

(Difference between revisions)
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'''procedure'''
'''procedure'''
-
see protocol3
+
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
 +
 
===Experiment:Electrophoresis of bcsC,D('''A.xylinum'''),bcsA,B,C,EFG,Q('''E.coli'''),and pSB13===
===Experiment:Electrophoresis of bcsC,D('''A.xylinum'''),bcsA,B,C,EFG,Q('''E.coli'''),and pSB13===
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*bcsC,D(''A.xylinum''),bcsA,B,C,EFG,Q(''E.coli''),pSB13
*bcsC,D(''A.xylinum''),bcsA,B,C,EFG,Q(''E.coli''),pSB13
-
If you want to know other materials, see protocol4
+
If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
 +
 
'''procedure'''
'''procedure'''
-
see protocol4
+
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
 +
 
===Experiment:Miniprep of BBa_K145001===
===Experiment:Miniprep of BBa_K145001===
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*Preculture of ''E.coli'' having BBa_K145001
*Preculture of ''E.coli'' having BBa_K145001
-
If you want to know other materials, see protocol9
+
If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol9</a></html>
 +
 
'''procedure'''
'''procedure'''
-
see protocol9
+
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol9</a></html>
 +
 
===Experiment:Ligation of pSB1C3 and bcsC,D(''A.xylinum''),bcsA(''E.coli'')===
===Experiment:Ligation of pSB1C3 and bcsC,D(''A.xylinum''),bcsA(''E.coli'')===
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*pSB1C3  
*pSB1C3  
*bcsC,D(''A.xylinum''),bcsA(''E.coli'')  
*bcsC,D(''A.xylinum''),bcsA(''E.coli'')  
-
If you want to know other materials, see protocol7
+
If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html>
 +
 
'''procedure'''
'''procedure'''
-
see protocol7
+
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
 +
 
===Experiment:Transformation of parts of bcsC,D(''A.xylinum''),bcsA(''E.coli'')===
===Experiment:Transformation of parts of bcsC,D(''A.xylinum''),bcsA(''E.coli'')===
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* parts of bcsC,D(''A.xylinum''),bcsA(''E.coli'')
* parts of bcsC,D(''A.xylinum''),bcsA(''E.coli'')
-
If you want to know other materials, see protocol8
+
If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
 +
 
'''procedure'''
'''procedure'''
-
see protocol8
+
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html>
 +
 
===Experiment:Electrophoresis of bcsA('''A.xylinum''')===
===Experiment:Electrophoresis of bcsA('''A.xylinum''')===
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*bcsA(''A.xylinum'')
*bcsA(''A.xylinum'')
-
If you want to know other materials, see protocol4
+
If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
 +
 
'''procedure'''
'''procedure'''
-
see protocol4
+
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
 +
 
'''result'''
'''result'''
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*bcsA(''A.xylinum'') in agarose gel
*bcsA(''A.xylinum'') in agarose gel
-
If you want to know other materials, see protocol5
+
If you want to know other materials, see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html>
 +
 
'''procedure'''
'''procedure'''
-
see protocol5
+
<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>

Revision as of 08:23, 24 October 2010


E.coli Fiber Project Notebook

August 2010
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Contents

2010/10/03 Sunday (Naoto)

Experiment:PCR of bcsA(A.xylinum)

member

naoto and watachin

marerial

  • bcsA(A.xylinum)

If you want to know other materials, see protocol3

procedure

see protocol3


Experiment:Electrophoresis of bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),and pSB13

member

naoto and watachin

marerial

  • bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),pSB13

If you want to know other materials, see protocol4


procedure

see protocol4


Experiment:Miniprep of BBa_K145001

member

naoto and watachin

marerial

  • Preculture of E.coli having BBa_K145001

If you want to know other materials, see protocol9


procedure

see protocol9


Experiment:Ligation of pSB1C3 and bcsC,D(A.xylinum),bcsA(E.coli)

member

naoto and watachin

marerial

  • pSB1C3
  • bcsC,D(A.xylinum),bcsA(E.coli)

If you want to know other materials, see protocol7


procedure

see protocol4


Experiment:Transformation of parts of bcsC,D(A.xylinum),bcsA(E.coli)

member

naoto and watachin

marerial

  • parts of bcsC,D(A.xylinum),bcsA(E.coli)

If you want to know other materials, see protocol8


procedure

see protocol8


Experiment:Electrophoresis of bcsA(A.xylinum)

member

naoto and watachin

marerial

  • bcsA(A.xylinum)

If you want to know other materials, see protocol4


procedure

see protocol4


result

Band of bcsA could be seen clearly

Experiment:Purification of bcsA(A.xylinum)

member

naoto and watachin

marerial

  • bcsA(A.xylinum) in agarose gel

If you want to know other materials, see protocol5


procedure

protocol4
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