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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/07
From 2010.igem.org
(Difference between revisions)
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| - | ==Agarose Gels Electro-phoresis== | + | ===Agarose Gels Electro-phoresis=== |
| - | + | '''member''' | |
bambi75 and watachin | bambi75 and watachin | ||
| - | + | '''Materials''' | |
*10×Loading Buffer 30μl | *10×Loading Buffer 30μl | ||
*DNA maker 15μl | *DNA maker 15μl | ||
| - | + | '''Procedure''' | |
| - | + | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | |
| + | ===collect DNA=== | ||
| - | + | '''member''' | |
| - | + | ||
| - | + | ||
| - | + | ||
Same above | Same above | ||
| - | + | '''Materials''' | |
*QB Buffer 1800μl | *QB Buffer 1800μl | ||
*PE Buffer 4200μl | *PE Buffer 4200μl | ||
*EB 300μl | *EB 300μl | ||
| - | + | '''Procedure''' | |
| - | + | <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html> | |
Revision as of 20:59, 23 October 2010
E.coli Fiber Project Notebook
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Agarose Gels Electro-phoresis
member bambi75 and watachin
Materials
- 10×Loading Buffer 30μl
- DNA maker 15μl
Procedure see protocol4
collect DNA
member Same above
Materials
- QB Buffer 1800μl
- PE Buffer 4200μl
- EB 300μl
Procedure
protocol5
