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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13
From 2010.igem.org
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'''Procedure''' | '''Procedure''' | ||
#mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products. | #mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products. | ||
| - | #incubation(37℃). | + | #incubation(37℃).<br /> |
Revision as of 20:30, 23 October 2010
E.coli Fiber Project Notebook
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2010/08/13 Friday (bambi75)
Experiment:Digestion of bcs
member
NEX , bambi75 and watachin
Materials
- PCR products 50μl×3
- m buffer 15μl
- BSA 15μl
- Xba1 3μl
- Spe1 3μl
Procedure
- mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
- incubation(37℃).
