Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13

From 2010.igem.org

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'''Procedure'''
'''Procedure'''
#mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
#mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
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#incubation(37℃).
+
#incubation(37℃).<br />

Revision as of 20:30, 23 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
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12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
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10 11 12 13 14 15 16
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31

2010/08/13 Friday (bambi75)

Experiment:Digestion of bcs

member

NEX , bambi75 and watachin


Materials

  • PCR products 50μl×3
  • m buffer 15μl
  • BSA 15μl
  • Xba1 3μl
  • Spe1 3μl


Procedure

  1. mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and Spe1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
  2. incubation(37℃).