Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/24

From 2010.igem.org

(Difference between revisions)
(New page: =August 24th (bambi75)= ==Agarose Gels Electro-phoresis== ===member=== NEX , bambi75 and watachin ===Materials,Procedure=== Same protocol on August 16th. PCR Product of insert are bcsA...)
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=August 24th (bambi75)=
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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==2010/08/24 Tuesday(bambi75)==
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==Agarose Gels Electro-phoresis==
 
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===member===
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==Experiment:Agarose Gels Electrophoresis==
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'''member'''
NEX , bambi75 and watachin
NEX , bambi75 and watachin
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===Materials,Procedure===
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'''Materials,Procedure'''
Same protocol on August 16th.
Same protocol on August 16th.
PCR Product of insert are bcsA and bcsC.
PCR Product of insert are bcsA and bcsC.
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==Experiment:PCR==
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==Medium making==
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'''member'''
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===member===
 
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easily and naoto
 
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===Materials , Procedure===
 
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Same protocol on August 10th.
 
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==PCR==
 
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===member===
 
NEX , bambi75 and watachin
NEX , bambi75 and watachin
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===materials===
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'''material'''
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*sterilized water 213μl
*sterilized water 213μl
*Ex taq buffer 30μl
*Ex taq buffer 30μl
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*K12bcsA antisense 15μl
*K12bcsA antisense 15μl
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===Procedure===
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'''Procedure'''
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Same on August 23th.
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Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
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==Restriction enzyme digestion==
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===Experiment:Restriction enzyme digestion===
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'''member'''
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===member===
 
Same above
Same above
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===Materials===
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'''Materials'''
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*PCR product
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*PCR production
*m buffer 30μl
*m buffer 30μl
*BSA 30μl
*BSA 30μl
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*SPE1 6μl
*SPE1 6μl
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===Procedure===
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'''Procedure'''
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Same on August 13th.
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Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 restriction enzyme digestion">protocol4</a></html>
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==Transformation==
 
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===member===
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==Experiment:Transformation==
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'''member'''
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Same above
Same above
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===Materials , Procedure===
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'''Materials , Procedure'''
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Same on August 17th.
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Please refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 trasnformation">protocol9</a></html>

Revision as of 18:39, 22 October 2010


E.coli Fiber Project Notebook

August 2010
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Contents

2010/08/24 Tuesday(bambi75)

Experiment:Agarose Gels Electrophoresis

member NEX , bambi75 and watachin

Materials,Procedure Same protocol on August 16th. PCR Product of insert are bcsA and bcsC.

Experiment:PCR

member

NEX , bambi75 and watachin

material

  • sterilized water 213μl
  • Ex taq buffer 30μl
  • dNTP 24μl
  • Ex taq 3μl
  • K12bcsA sense 15μl
  • K12bcsA antisense 15μl

Procedure Please see protocol3


Experiment:Restriction enzyme digestion

member

Same above

Materials

  • PCR production
  • m buffer 30μl
  • BSA 30μl
  • Xba1 6μl
  • SPE1 6μl

Procedure

Please see protocol4


Experiment:Transformation

member

Same above

Materials , Procedure

Please refer to protocol9
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