Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13

From 2010.igem.org

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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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===Experiment:restriction enzyme digestion===
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==2010/08/13 Friday (bambi75)==
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===Experiment:Digestion===
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'''member'''
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====member====
 
NEX , bambi75 and watachin
NEX , bambi75 and watachin
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===Materials===
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'''Materials'''
*PCR products  50μl×3
*PCR products  50μl×3
*m buffer  15μl
*m buffer  15μl
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*BSA 1 15μl
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*BSA 15μl
*Xba1  3μl
*Xba1  3μl
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*SPE1 3μl
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*Spe1 3μl
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===Equipment===
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*pipet
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*pipet tip
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===Procedure===
 
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1)mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and SPE1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
 
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2)incubation(37℃).
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'''Procedure'''
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#mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and SPE1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
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#incubation(37℃).

Revision as of 18:16, 22 October 2010


E.coli Fiber Project Notebook

August 2010
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October 2010
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2010/08/13 Friday (bambi75)

Experiment:Digestion

member

NEX , bambi75 and watachin


Materials

  • PCR products 50μl×3
  • m buffer 15μl
  • BSA 15μl
  • Xba1 3μl
  • Spe1 3μl


Procedure

  1. mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and SPE1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
  2. incubation(37℃).
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