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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13
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===Experiment:restriction enzyme digestion=== | ===Experiment:restriction enzyme digestion=== | ||
Revision as of 17:34, 22 October 2010
E.coli Fiber Project Notebook
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Contents |
Experiment:restriction enzyme digestion
member
NEX , bambi75 and watachin
Materials
- PCR products 50μl×3
- m buffer 15μl
- BSA 1 15μl
- Xba1 3μl
- SPE1 3μl
Equipment
- pipet
- pipet tip
Procedure
1)mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and SPE1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
2)incubation(37℃).
