Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/22

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==2010/09/22 Wednesday (Naoto)==
==2010/09/22 Wednesday (Naoto)==

Revision as of 03:40, 24 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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Contents

2010/09/22 Wednesday (Naoto)

Experiment:PCR bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

member

naoto and NEX

material

  • bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

you can see other materials at protocol3

procedure

protocol3

Experiment:Insert　check PCR

member

naoto and NEX

material

  • Colonies of E.coli having bcsA,B(A.xylinum)

you can see other materials at protocol3

procedure

protocol3

Experiment:Electrophoresis of bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C3

member

naoto and NEX

material

  • PCR production (bcsABCD,bcsB(A.xylinum),bcsA,B,C(E.coli) and pSB1C)

If you want to know other materials,refer to protocol4

procedure

see protocol4

result

Bands of bcsB(A.xylinum) and bcsA,B,C(E.coli) could be seen

Experiment:miniprep parts of bcsB,C(E.coli)

member

naoto and NEX

material

  • bcsB,C(E.coli) in pSB1C3

If you want to know other materials,refer to protocol9

procedure

see protocol9

Experiment:Insert　check PCR

member

naoto and NEX

material

  • Insert check PCR production (bcsA,B(A.xylinum))

you can see other materials at protocol3

procedure

protocol3

result

a band of No.41(bcsB,Axylinum) appeared slightly

Experiment:Purification of bcsB(A.xylinum) and bcsA,B,C(E.coli) with QIAGEN

member

naoto and NEX

material

  • bcsB(A.xylinum) and bcsA,B,C(E.coli) in agarose gel

you can see other materials at protocol5

procedure

see protocol5

Experiment:digestion of bcsB(A.xylinum) and bcsA,B,C(E.coli)

member

naoto and NEX

material

  • bcsB(A.xylinum) and bcsA,B,C(E.coli)

you can see other materials at protocol6

procedure

see protocol6

Experiment:Subculture E.coli having bcsB(A.xylinum)

member

naoto and NEX

material

  • LB+Cam　Broth
  • a colony of E.coli having bcsB(A.xylinum)

you can see other materials at protocol1

procedure

see protocol1
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