"
Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/09
From 2010.igem.org
(Difference between revisions)
(→Experiment:Electrophoresis) |
|||
| Line 1: | Line 1: | ||
| - | {{:Team:Tokyo_Metropolitan/ | + | {{:Team:Tokyo_Metropolitan/Notebook/Fiber}} |
==2010/09/09 Thursday (Naoto)== | ==2010/09/09 Thursday (Naoto)== | ||
===Experiment:Ligation=== | ===Experiment:Ligation=== | ||
Revision as of 17:37, 22 October 2010
E.coli Fiber Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010/09/09 Thursday (Naoto)
Experiment:Ligation
member
naoto and NEX
material
- bcsA,B(from A.xylinum) 10µl each
- bcsC,D(from A.xylinum) 10µl each
- bcsA,B(from E.coli) 10µl each
- ligation Mix(nippon gene) 20µl each
procedure
- add material to PCR tube
- incubate (20℃ 10min)
Experiment:Electrophoresis
member
naoto and NEX
material
- ligation products(see above)
we show other material at protocol4
procedure
follow protocol4
