Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/11

From 2010.igem.org

(Difference between revisions)

Revision as of 17:38, 22 October 2010

E.coli Fiber Project Notebook

2010/8/11 Wednesday (Naoto)

Experiment:Grow up a culture of A.xylinum

Member

easily and naoto

Material

A.xylinum JCM strain 7644
medium for A.xylinum
ｒecovery solution for A.xylinum

Procedure

see protocol1

Experiment:Grow up a culture of E.coli

Member

NEX,Bambi75 and watachin

Material

see protocol2

Procedure

see protocol2

@@ Line 1: / Line 1: @@
-{{:Team:Tokyo_Metropolitan/Header}}
+{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
 ==2010/8/11 Wednesday (Naoto)==
 ===Experiment:Grow up a culture of ''A.xylinum''===
-'''Member'''<br />
+'''Member'''
 easily and naoto
-'''Materials'''
+'''Material'''
 *''A.xylinum'' JCM strain 7644
-*media for A.xylinum(Open Wet Ware recommended)
+*medium for A.xylinum
-*media for A.xylinum(JCM recommended) 100ml
+*ｒecovery solution for ''A.xylinum''
- glucoce 10g
- yeast extract 1g
- CaCO3 3g
- DW 100ml
- (add agar 1.5g for plates)
-*ｒecovery solution for ''A.xylinum'' 100ml
-  peptone 0.5g
- yeast extract 0.3g
- DW 100ml
 '''Procedure'''
-#prepared media (plates of Open Wet Ware recommended media were made yesterday)
+see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow_up_a_culture_of_A.xylinum">protocol1</a></html>
-#autoclaved to sterilize media(121°C　20minute)
-#added ｒecovery solution to an ampule which had ''A.xylinum''
-#streaked ''A.xylinum'' onto plates or in media
-#incubated at 26°C
 ===Experiment:Grow up a culture of ''E.coli''===
-'''Member'''<br />
+'''Member'''
 NEX,Bambi75 and watachin
-'''Materials'''<br />
+'''Material'''
-LB plates
+see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow_up_a_culture_of_E.coli">protocol2</a></html>
 '''Procedure'''
-#streaked ''E.coli'' onto plates
-#incubated at 37°C
+see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Grow_up_a_culture_of_E.coli">protocol2</a></html>