Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/15
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Latest revision as of 18:10, 24 October 2010
E.coli Pattern Formation Project Notebook
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2010/08/15
1:DNA extraction with ISOPLANT(NIPPON GENE)
<Member>
Mariko, easily, nito, Hitomi, Taka
<Sample>
・E-coli (having BBa_I732901 plasmid)
<Protocol>
SeeProtocol 5
2:DNA concentration measurement
<Member>
Mariko, easily, nito, Hitomi, Taka
<Materials, Sample>
・TE
・E-coli (having BBa_I732901 plasmid)
<Protocol>
- Add 2µl of TE and 2µl of sample into the tube, and mix it gently.
- Fall in drops 1 on the measuring machine.
<Result>
Concentration: 16.8
A320: 0.046
A260/A280: 3.414
A260/A230: 0.062