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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/29
From 2010.igem.org
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*bcsC,D(''A.xylinum'') in colonies of '''E.coli''' | *bcsC,D(''A.xylinum'') in colonies of '''E.coli''' | ||
| - | If you want to know other materials,refer to protocol3 | + | If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3] |
'''procedure''' | '''procedure''' | ||
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*bcsB(''E.coli'') | *bcsB(''E.coli'') | ||
| - | If you want to know other materials,refer to protocol6 | + | If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6] |
'''procedure''' | '''procedure''' | ||
| - | follow to protocol6 | + | follow to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol6:DNA_Purification_with_silica_gel protocol6] |
===Experiment:subculture of ''A.xylinum''=== | ===Experiment:subculture of ''A.xylinum''=== | ||
| Line 69: | Line 69: | ||
*parts of bcsA,B(''A.xylinum'') | *parts of bcsA,B(''A.xylinum'') | ||
| - | If you want to know other materials,refer to protocol8 | + | If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol8:Ligation protocol8] |
'''procedure''' | '''procedure''' | ||
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*PCR production (bcsC,D(''A.xylinum'') ) | *PCR production (bcsC,D(''A.xylinum'') ) | ||
| - | If you want to know other materials,refer to protocol4 | + | If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4] |
'''procedure''' | '''procedure''' | ||
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*PCR production (PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(''E.coli'')) | *PCR production (PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(''E.coli'')) | ||
| - | If you want to know other materials,refer to protocol4 | + | If you want to know other materials,refer to [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4] |
'''procedure''' | '''procedure''' | ||
Latest revision as of 16:05, 27 October 2010
E.coli Fiber Project Notebook
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2010/09/29 Wednesday (Naoto)
Experiment:Insert check PCR
member
naoto and NEX
material
- bcsC,D(A.xylinum) in colonies of E.coli
If you want to know other materials,refer to protocol3
procedure
see protocol3
Experiment:PCR of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli),bcsQ(E.coli),RBS-T7polymerase
member
naoto and NEX
material
- A colony of A.xylinum(for templete DNA)
- bcsE~G(E.coli)
- bcsQ(E.coli)
- T7polymerase(BBa_K145001)
If you want to know other materials,refer to protocol3
procedure
see protocol3
Experiment:digestion of bcsB(E.coli)
member
naoto and easily
material
- bcsB(E.coli)
If you want to know other materials,refer to protocol6
procedure
follow to protocol6
Experiment:subculture of A.xylinum
member
naoto and easily
material
see protocol1 A.xylinum(subcultured at 9/1)
procedure
see protocol1
Experiment:Transformation
member
naoto and easily
material
- parts of bcsA,B(A.xylinum)
If you want to know other materials,refer to protocol8
procedure
see protocol8
Experiment:Electrophoresis of insert check PCR
member
naoto
material
- PCR production (bcsC,D(A.xylinum) )
If you want to know other materials,refer to protocol4
procedure
see protocol4
result
Bands(102 and 104) of bcsD(A.xylinum) could be seen slightly
So we prepared precultures for miniprep
Experiment:Electrophoresis of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli)
member
naoto
material
- PCR production (PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli))
If you want to know other materials,refer to protocol4
procedure
see protocol4
result
All bands don't have collect length.
