Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/24
From 2010.igem.org
(Difference between revisions)
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:'''Materials and Procedure''' | :'''Materials and Procedure''' | ||
- | : | + | :templete DNA (bcsA,B,C,D) |
- | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR"> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html> |
===Experiment:Colony PCR=== | ===Experiment:Colony PCR=== | ||
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:'''Materials and Procedure''' | :'''Materials and Procedure''' | ||
- | : | + | :E.coli induced bcsA,B,C and D |
- | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html> | |
- | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR"> | + | |
===Experiment:Ligation=== | ===Experiment:Ligation=== | ||
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:'''Materials and Procedure''' | :'''Materials and Procedure''' | ||
- | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation"> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Ligation">protocol3</a></html> |
===Experiment:Digestion=== | ===Experiment:Digestion=== | ||
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:'''Materials and Procedure''' | :'''Materials and Procedure''' | ||
- | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion"> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">protocol7</a></html> |
===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
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:'''Materials and Procedure''' | :'''Materials and Procedure''' | ||
- | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis"> | + | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html> |
:'''Result''' | :'''Result''' |
Latest revision as of 14:33, 27 October 2010
E.coli Fiber Project Notebook
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2010/9/24 Friday (watachin)
Experiment:PCR
- Member
- easily
- Materials and Procedure
- templete DNA (bcsA,B,C,D)
- follow protocol3
Experiment:Colony PCR
- Member
- watachin
- Materials and Procedure
- E.coli induced bcsA,B,C and D
- follow protocol3
Experiment:Ligation
- Member
- easily
- Materials and Procedure
- follow protocol3
Experiment:Digestion
- Member
- watachin
- Materials and Procedure
- follow protocol7
Experiment:Electrophoresis
- Member
- easily and watachin
- Materials and Procedure
- follow protocol4
- Result
Experiment:Transformation
- Member
- easily and watachin
- Materials and Procedure
- follow protocol9