Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/23

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:plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
:plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
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===Experiment:Insert check PCR===
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===Experiment:Colony PCR===
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:Colony PCR production of bcs genes
:Colony PCR production of bcs genes
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:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html>
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:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
===Experiment:Insert check Electrophoresis===
===Experiment:Insert check Electrophoresis===

Revision as of 14:25, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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2010/9/23 Thursday (watachin)

Experiment:PCR

Member
naoto
Materials and Procedure
E.coli introduced bcsA,B,C,D
follow protocol3

Experiment:Plating

Member
watachin
Materials and Procedure
plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth

Experiment:Colony PCR

Member
naoto
Materials and Procedure
Colony PCR production of bcs genes
follow protocol3

Experiment:Insert check Electrophoresis

Member
watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Result

2010-09-23-01.jpg 2010-09-23-02.jpg

Experiment:Electrophoresis of bcs genes

Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)

Experiment:DNA Extraction from agarose gel

Member
naoto and watachin
Materials and Procedure
follow protocol5

Experiment:Electrophoresis

Member
naoto and watachin
Materials and Procedure
  • Colony PCR production
follow the protocol(Electrophoresis)
Result

2010-09-23-03.jpg

Experiment:Transformation

Member
watachin
Materials and Procedure
follow the protocol(Transformation)

Experiment:Electrophoresis

Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Result

2010-09-23-04.jpg

Experiment:DNA Extraction from agarose gel

Member
naoto and watachin
Materials and Procedure
follow protocol5
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