Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/26

(Difference between revisions)

Latest revision as of 13:56, 27 October 2010

E.coli Fiber Project Notebook

Member

NEX , Bambi75 and watachin

Materials

Procedure

①mix all materials for 4 tubes.

②elongation

※30cycle ☆ to ★.

@@ Line 3: / Line 3: @@
 ===Experiment:PCR===
-'''member'''
+:'''Member'''
-NEX , Bambi75 and watachin
+:NEX , Bambi75 and watachin
-'''Materials'''
+:'''Materials'''
-*2×PCR buffer  25×4μl
+:*2×PCR buffer  25×4μl
-*2mM dNTP  10×4μl
+:*2mM dNTP  10×4μl
-*10mM primer(sense)bcsA,B,C and D 2.5μl each
+:*10mM primer(sense)bcsA,B,C and D 2.5μl each
-*10mM primer(antisense)bcsA,B,C and D 2.5μl each
+:*10mM primer(antisense)bcsA,B,C and D 2.5μl each
-*template DNA a little
+:*template DNA a little
-*Q water 9×4μl
+:*Q water 9×4μl
-*KOD FX 0.5×4μl
+:*KOD FX 0.5×4μl
-'''Procedure'''
+:'''Procedure'''
-①mix all materials for 4 tubes.
+:①mix all materials for 4 tubes.
-②elongation
+:②elongation
-*bcsA,bcsB and bcsC
+:*bcsA,bcsB and bcsC
-**94℃ 2min
+:**94℃ 2min
-**98℃ 10sec☆
+:**98℃ 10sec☆
-**55℃ 30sec
+:**55℃ 30sec
-**68℃ 4min★
+:**68℃ 4min★
-**68℃ 7min
+:**68℃ 7min
-**10℃　∞
+:**10℃　∞
-*bcsD
+:*bcsD
-**94℃ 2min
+:**94℃ 2min
-**98℃ 10sec☆
+:**98℃ 10sec☆
-**55℃ 30sec
+:**55℃ 30sec
-**68℃ 1min★
+:**68℃ 1min★
-**68℃ 7min
+:**68℃ 7min
-**10℃　∞
+:**10℃　∞
-※30cycle ☆ to ★.
+:※30cycle ☆ to ★.
 <br/>