Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/21
From 2010.igem.org
(Difference between revisions)
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===Experiment:DNA purification from agarose gel=== | ===Experiment:DNA purification from agarose gel=== | ||
- | '''Member'''<br /> | + | :'''Member'''<br /> |
:watachin | :watachin | ||
- | '''Materials and Procedure''' | + | :'''Materials and Procedure''' |
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html> | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html> | ||
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===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
- | '''Member'''<br /> | + | :'''Member'''<br /> |
:NEX and watachin | :NEX and watachin | ||
- | '''Materials and Procedure''' | + | :'''Materials and Procedure''' |
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol5</a></html> | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol5</a></html> | ||
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[[Image:2010-09-21-03.jpg]] | [[Image:2010-09-21-03.jpg]] | ||
- | ===Experiment:DNA | + | ===Experiment:DNA Extraction from agarose gel=== |
- | '''Member'''<br /> | + | :'''Member'''<br /> |
:NEX and watachin | :NEX and watachin | ||
- | '''Materials and Procedure''' | + | :'''Materials and Procedure''' |
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html> | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html> | ||
===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
- | '''Member'''<br /> | + | :'''Member'''<br /> |
:watachin | :watachin | ||
- | '''Materials and Procedure''' | + | :'''Materials and Procedure''' |
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html> | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html> | ||
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===Experiment:DNA purification from agarose gel with QIAGEN=== | ===Experiment:DNA purification from agarose gel with QIAGEN=== | ||
- | '''Member'''<br /> | + | :'''Member'''<br /> |
:watachin | :watachin | ||
- | '''Materials and Procedure''' | + | :'''Materials and Procedure''' |
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html> | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html> | ||
===Experiment:Transformation=== | ===Experiment:Transformation=== | ||
- | '''Member'''<br /> | + | :'''Member'''<br /> |
:watachin | :watachin | ||
- | '''Materials and Procedure''' | + | :'''Materials and Procedure''' |
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html> | :follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html> |
Revision as of 13:58, 27 October 2010
![](https://static.igem.org/mediawiki/2010/9/9f/Re-main.png)
E.coli Fiber Project Notebook
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2010/9/21 Tuesday (watachin)
Experiment:Miniprep (extraction of plasmid kit)
- Member
- easily and NEX
- Materials and Procedure
- follow protocol10
Experiment:PCR
- Member
- watachin
- Materials and Procedure
- PCR of pSB1C3
- follow protocol3
Experiment:Electrophoresis
Member
- easily
Materials and Procedure
- follow protocol4
Result
Experiment:DNA purification from agarose gel
- Member
- watachin
- Materials and Procedure
- follow protocol5
Experiment:Digestion
Member
- NEX and watachin
Materials and Procedure
- follow protocol7
Experiment:Electrophoresis
- Member
- NEX and watachin
- Materials and Procedure
- follow protocol5
Result
Experiment:DNA Extraction from agarose gel
- Member
- NEX and watachin
- Materials and Procedure
- follow protocol5
Experiment:Electrophoresis
- Member
- watachin
- Materials and Procedure
Result
Experiment:DNA purification from agarose gel with QIAGEN
- Member
- watachin
- Materials and Procedure
Experiment:Transformation
- Member
- watachin
- Materials and Procedure
- follow the protocol(Transformation)