Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/18

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:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
:refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
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:Bands of bcsB,C(''A.xylinum'') appreared
:Bands of bcsB,C(''A.xylinum'') appreared

Revision as of 07:10, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/09/18 Saturday (Naoto)

Experiment:Electrophoresis

Member
naoto and easily
Material
  • PCR production(pSB1C3)
If you want to know other materials,refer to protocol4
Procedure
follow to protocol3
Result
Bands didn't appear

Experiment:Colony PCR

Member
naoto and easily
Material
  • Parts of bcsA,B,C,D(A.xylinum) bcsA,B,C(E.coli)in colonies of E,coli
If you want to know other materials,refer to protocol3
Procedure
follow to protocol3

Experiment:PCR

Member
naoto and easily
Material
  • bcsA,B,C,D(A.xylinum),pSB1C3
If you want to know other materials,refer to protocol3
Procedure
follow to protocol3

Experiment:Electrophoresis of bcsA,B,C,D(A.xylinum),pSB1C3

Member
naoto and easily
Material
  • PCR production(bcsA,B,C,D(A.xylinum),bcsA,B,C(E.coli),pSB1C3)
If you want to know other materials,refer to protocol4
Procedure
follow to protocol3
Result
Only band of bcsA(E.coli) could be seen

Experiment:DNA Extraction from agarose gel

Member
naoto and easily
Material
  • bcsA(E.coli) in agarose gel
If you want to know other materials,refer to protocol5
Procedure
follow to protocol5

Experiment:Electrophoresis of Colony PCR

Member
naoto and easily
Material
  • PCR production (parts of bcsA,B,C,D(A.xylinum) and bcsA(E.coli)in colonies of E,coli)
If you want to know other materials,refer to protocol4
procedure
refer to protocol4
Result
Bands of bcsB,C(A.xylinum) appreared
  • 1~8 bcsA(A.xylinum)
  • 9~12 bcsB(A.xylinum)
  • 13 bcsC(A.xylinum)
  • 14~15 bcsD(A.xylinum)
  • 16~20 bcsA(E.coli)

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