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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/24
From 2010.igem.org
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===Experiment:PCR=== | ===Experiment:PCR=== | ||
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:NEX , bambi75 and watachin | :NEX , bambi75 and watachin | ||
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:*sterilized water 213μl | :*sterilized water 213μl | ||
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:Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | :Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | ||
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===Experiment:Restriction enzyme digestion=== | ===Experiment:Restriction enzyme digestion=== | ||
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:Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 restriction enzyme digestion">protocol7</a></html> | :Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80 restriction enzyme digestion">protocol7</a></html> | ||
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===Experiment:Transformation=== | ===Experiment:Transformation=== | ||
Latest revision as of 21:45, 26 October 2010
E.coli Fiber Project Notebook
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2010/08/24 Tuesday(bambi75)
Experiment:Agarose Gels Electrophoresis
- member
- NEX , bambi75 and watachin
- Materials,Procedure
- Same protocol on August 16th.
- PCR Product of insert are bcsA and bcsC.
Experiment:PCR
- Member
- NEX , bambi75 and watachin
- Material
- sterilized water 213μl
- Ex taq buffer 30μl
- dNTP 24μl
- Ex taq 3μl
- bcsA sense 15μl
- bcsA antisense 15μl
- Procedure
- Please see protocol3
Experiment:Restriction enzyme digestion
- Member
- Same above
- Materials
- PCR production
- M buffer 30μl
- BSA 30μl
- Xba1 6μl
- Spe1 6μl
- Procedure
- Please see protocol7
Experiment:Transformation
- Member
- Same above
- Materials , Procedure
- Please refer to protocol9
