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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/16
From 2010.igem.org
(Difference between revisions)
| Line 13: | Line 13: | ||
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html> | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80">protocol4</a></html> | ||
| + | |||
| + | ===Experiment:Digestion=== | ||
| + | '''material''' | ||
| + | *bcsD | ||
| + | *XbaI | ||
| + | *SpeI | ||
| + | you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html> | ||
| + | |||
| + | '''procedure''' | ||
| + | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol6</a></html> | ||
===Experiment:DNA purification with silica gel=== | ===Experiment:DNA purification with silica gel=== | ||
'''material''' | '''material''' | ||
| - | *PCR production(bcsD | + | *PCR production(bcsD) |
you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | ||
| Line 22: | Line 32: | ||
refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5-2</a></html> | ||
| + | |||
| + | ===Experiment:Ligation=== | ||
| + | '''material''' | ||
| + | *bcsD | ||
| + | *pSB1C3 | ||
| + | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html> | ||
| + | |||
| + | '''procedure''' | ||
| + | |||
| + | refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol7</a></html> | ||
| + | |||
| + | ===Experiment:Transformation=== | ||
| + | '''material''' | ||
| + | *Competent cell(NovaBlue) | ||
| + | *bcsD in pSB1C3 | ||
| + | you can see materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> | ||
| + | |||
| + | '''procedure''' | ||
| + | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol8</a></html> | ||
| + | |||
<br/> | <br/> | ||
Revision as of 17:45, 26 October 2010
E.coli Fiber Project Notebook
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Contents |
2010/10/16 Saturday (Naoto)
member naoto and watachin
Experiment:Electrophoresis
material
- PCR production (bcsA,bcsB,bcsC and bcsD)
you can see other materials at protocol4
procedure
see protocol4
Experiment:Digestion
material
- bcsD
- XbaI
- SpeI
you can see other materials at protocol6
procedure see protocol6
Experiment:DNA purification with silica gel
material
- PCR production(bcsD)
you can see materials at protocol5-2
procedure
refer to protocol5-2
Experiment:Ligation
material
- bcsD
- pSB1C3
you can see materials at protocol7
procedure
refer to protocol7
Experiment:Transformation
material
- Competent cell(NovaBlue)
- bcsD in pSB1C3
you can see materials at protocol8
procedure see protocol8
