Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/14

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refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
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Revision as of 17:07, 26 October 2010


E.coli Fiber Project Notebook

August 2010
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Contents

2010/10/14 Thursday (Naoto)

member

naoto,maki and watachin

Preparation:Measure DNA concentration

Material

  • bcsA
  • bcsB
  • bcsC
  • bcsD
  • pSB1C3
  • TE buffer

Procedure

  1. mix 4μl of DNA and 4μl of TE buffer
  2. set mixture in absorption spectrometer
  3. measure DNA concentrations

Experiment:PCR

material

  • templete DNA (from A.xylinum and pSB1C3)

you can see other materials at protocol3

procedure

see protocol3

Experiment:Electrophoresis

material

  • bcsA
  • bcsB
  • bcsC
  • bcsD

you can see materials at protocol4

procedure

refer to protocol4


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