Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/20

From 2010.igem.org

(Difference between revisions)
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'''material'''
'''material'''
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*bcsB
+
*bcsB:1~24
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*bcsC
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*bcsC:25~48
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other materials were same as <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
+
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
'''procedure'''
'''procedure'''
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see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
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===Experiment:Sequence===
+
===Experiment:Electrophoresis===
'''material'''
'''material'''
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*bcsD in pSB1C3(No.89~96)
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*PCR production (Colony PCR)
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*Big Dye
+
you can see other materials at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
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*primer
+
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*DW
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*ethanol
+
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*EDTA
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'''procedure'''
'''procedure'''
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#mix materials(DNA<50ng)
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refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80electrophoresis">protocol4</a></html>
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#PCR
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#Add EDTA and Ethanol and put at room temperature　(15min)
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#centrifuge (8000rpm,30min)
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#throw away supernatant
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#add ethanol again
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#centrifuge (8000rpm,15min)
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#put at refrigerator
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#throw away supernatant
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#add Hi-Di solution
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#transfer these sample to plate for sequence
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#read sequence
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'''result'''
'''result'''
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Failure to read sequence
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We couldn't see bands of bcsB and bcsC...
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+
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<br/>
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Revision as of 18:03, 26 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/10/20 Wednesday (Naoto)

member naoto and watachin

Experiment:Colony PCR

material

  • bcsB:1~24
  • bcsC:25~48

you can see other materials at protocol3

procedure

see protocol3

Experiment:Electrophoresis

material

  • PCR production (Colony PCR)

you can see other materials at protocol4

procedure

refer to protocol4

result

We couldn't see bands of bcsB and bcsC...
Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/10/20"