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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/23
From 2010.igem.org
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html> | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html> | ||
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===Experiment:Plating=== | ===Experiment:Plating=== | ||
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plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth | plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth | ||
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===Experiment:Insert check PCR=== | ===Experiment:Insert check PCR=== | ||
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'''Result''' | '''Result''' | ||
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===Experiment:DNA purification from agarose gel with QIAGEN=== | ===Experiment:DNA purification from agarose gel with QIAGEN=== | ||
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'''Materials and Procedure''' | '''Materials and Procedure''' | ||
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html> | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html> | ||
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===Experiment:Insert check Electrophoresis=== | ===Experiment:Insert check Electrophoresis=== | ||
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'''Materials and Procedure''' | '''Materials and Procedure''' | ||
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html> | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html> | ||
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===Experiment:Electrophoresis=== | ===Experiment:Electrophoresis=== | ||
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'''Materials and Procedure''' | '''Materials and Procedure''' | ||
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html> | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html> | ||
Revision as of 14:19, 27 October 2010
E.coli Fiber Project Notebook
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2010/9/23 Thursday (watachin)
Experiment:PCR
Member
naoto
Materials and Procedure
Direct PCR of bcs genes
follow the protocol(Direct PCR)
Experiment:Plating
Member
watachin
Materials and Procedure
plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
Experiment:Insert check PCR
Member
naoto
Materials and Procedure
Insert check of bcs genes
follow the protocol(Direct PCR)
Experiment:Insert check Electrophoresis
Member
watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Result
Experiment:Electrophoresis of bcs genes
Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Result
Experiment:DNA purification from agarose gel with QIAGEN
Member
naoto and watachin
Materials and Procedure
follow the protocol(DNA purification from agarose gel with QIAGEN)
Experiment:Insert check Electrophoresis
Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Result
Experiment:Transformation
Member
watachin
Materials and Procedure
follow the protocol(Transformation)
Experiment:Electrophoresis
Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Result
Experiment:DNA purification from agarose gel with QIAGEN
Member
naoto and watachin
Materials and Procedure
follow the protocol(DNA purification from agarose gel with QIAGEN)
