Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16

From 2010.igem.org

(Difference between revisions)
Line 59: Line 59:
*bcsA,B,C and D(A.xylium)
*bcsA,B,C and D(A.xylium)
*pSB1C3
*pSB1C3
 +
 +
 +
 +
 +
<br/>

Revision as of 17:15, 24 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

Contents

2010/09/16 Thuesday (Bambi75)

Experiment:PCR

member

naoto and bambi75

materials,Procedure

Same protocol4

  • 95℃3min
  • 96℃1min
  • 51℃1min
  • 72℃3min
  • 10℃∞

(96℃~72℃/30cycle)

Experiment:Agarose Gels Electro-phoresis

member

Same above

Materials,Procedure Same protocol on August 16th. PCR Product of insert is pBS1C3

Experiment:Experiment:Make Agarose Gels Electro-phoresis

Member

Same above

Materials and Procedure

Same protocol4

Preparation:Medium making

member

Same above

materials and procedure

Same protocol1


Experiment:Ligation

member

Same above

materials and procedure follow protocol8

  • bcsA,B and C(E.coli).
  • bcsA,B,C and D(A.xylium)
  • pSB1C3