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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/27
From 2010.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
==2010/9/27 Monday (watachin)(bambi75)== | ==2010/9/27 Monday (watachin)(bambi75)== | ||
| - | ==PCR/bcsA and D(A.xylium)/bcsA,B and C(E.coli)== | + | ===PCR/bcsA and D(A.xylium)/bcsA,B and C(E.coli)=== |
| - | + | '''member''' | |
watachin and bambi75 | watachin and bambi75 | ||
| - | + | '''materials,Procedure''' | |
Same on August 23th. | Same on August 23th. | ||
*95℃5min | *95℃5min | ||
| Line 17: | Line 17: | ||
（95℃～72℃/30cycle） | （95℃～72℃/30cycle） | ||
| - | ==Agarose Gels Electro-phoresis== | + | ===Agarose Gels Electro-phoresis=== |
| + | |||
| + | '''member''' | ||
| - | |||
Same above | Same above | ||
| - | + | ||
| - | Same protocol | + | '''Materials and Procedure''' |
| + | |||
| + | Same protocol <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80agarose gel electrophoresis">the protocol4</a></html> | ||
| + | |||
PCR Product of insert is the above things. | PCR Product of insert is the above things. | ||
| Line 28: | Line 32: | ||
===member=== | ===member=== | ||
| - | Same above | + | Same above |
===Materials and Procedure=== | ===Materials and Procedure=== | ||
| - | Same protocol | + | Same protocol <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80restriction enzyme digestion">the protocol6</a></html> |
| + | |||
==Agarose Gels Electro-phoresis== | ==Agarose Gels Electro-phoresis== | ||
Revision as of 04:09, 24 October 2010
E.coli Fiber Project Notebook
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Contents |
2010/9/27 Monday (watachin)(bambi75)
PCR/bcsA and D(A.xylium)/bcsA,B and C(E.coli)
member watachin and bambi75
materials,Procedure Same on August 23th.
- 95℃5min
- 95℃30sec
- 55℃30sec
- 72℃4min
- 72℃5min
- 10℃∞
（95℃～72℃/30cycle）
Agarose Gels Electro-phoresis
member
Same above
Materials and Procedure
Same protocol the protocol4
PCR Product of insert is the above things.
restriction enzyme digestion
member
Same above
Materials and Procedure
Same protocol the protocol6
Agarose Gels Electro-phoresis
member
Same above
Materials and Procedure
Same protocol on August 16th. PCR Product of insert is the above things.
Ligation
member
Same above
materials and procedure
Follow the protocol(Ligation).
- bcsB(E.coli)
- bcsC(E.coli)
Ligation and Transformation
member
Same above
materials and procedure
Same protocol on September 21st.
PCR/bcsQ and bcsEFG(E.coli)
member
watachin and bambi75
materials,Procedure
Same on August 23th.
- 95℃5min
- 95℃30sec
- 55℃30sec
- 72℃4min
- 72℃5min
- 10℃∞
（95℃～72℃/30cycle）
Agarose Gels Electro-phoresis
member
Same above
Materials and Procedure
Same protocol on August 16th. PCR Product of insert is the above things.
PCR
PCR of A.xylinum bcsA,D and E.coli bcsA,B,C ,pSB1C3
Electrophoreses of PCR production
Result
Digestion
bcsA → Xba,Spe
bcsB → Xba
bcsC → Spe
pSB1C3 → Xba, Spe
Electrophoreses of PCR productions
Result
DNA Purification
Ligation
DNA Purification
Ligation
Transfomation
PCR
Electrophoreses of PCR productions
Result
