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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/09
From 2010.igem.org
(Difference between revisions)
| Line 11: | Line 11: | ||
*bcsC,D(from ''A.xylinum'') 10µl each | *bcsC,D(from ''A.xylinum'') 10µl each | ||
*bcsA,B(from ''E.coli'') 10µl each | *bcsA,B(from ''E.coli'') 10µl each | ||
| - | * | + | *Ligation Covinience Kit 20µl each |
'''procedure''' | '''procedure''' | ||
| Line 26: | Line 26: | ||
*ligation products(see above) | *ligation products(see above) | ||
| - | we show other material at | + | we show other material at <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> |
'''procedure''' | '''procedure''' | ||
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html> | ||
Revision as of 21:09, 23 October 2010
E.coli Fiber Project Notebook
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2010/09/09 Thursday (Naoto)
Experiment:Ligation
member
naoto and NEX
material
- bcsA,B(from A.xylinum) 10µl each
- bcsC,D(from A.xylinum) 10µl each
- bcsA,B(from E.coli) 10µl each
- Ligation Covinience Kit 20µl each
procedure
- add material to PCR tube
- incubate (20℃ 10min)
Experiment:Electrophoresis
member
naoto and NEX
material
- ligation products(see above)
we show other material at protocol3
procedure
see protocol3
