Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/07

From 2010.igem.org

(Difference between revisions)
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==2010/09/07 Tuesday (Bambi75)==
==2010/09/07 Tuesday (Bambi75)==
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===Agarose Gels Electro-phoresis===
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===Experiment:Agarose Gels Electro-phoresis===
'''member'''
'''member'''
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see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html>
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===collect DNA===
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===Experiment:Extraction of DNA from agarose gel===
'''member'''
'''member'''

Revision as of 16:58, 24 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
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September 2010
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October 2010
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2010/09/07 Tuesday (Bambi75)

Experiment:Agarose Gels Electro-phoresis

member

bambi75 and watachin

Materials

  • 10×Loading Buffer 30μl
  • DNA maker 15μl

Procedure

see protocol4

Experiment:Extraction of DNA from agarose gel

member Same above

Materials

  • QB Buffer 1800μl
  • PE Buffer 4200μl
  • EB 300μl

(sum volume)

Procedure

refer to protocol5