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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/07
From 2010.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
'''member''' | '''member''' | ||
| + | |||
bambi75 and watachin | bambi75 and watachin | ||
'''Materials''' | '''Materials''' | ||
| + | |||
*10×Loading Buffer 30μl | *10×Loading Buffer 30μl | ||
*DNA maker 15μl | *DNA maker 15μl | ||
'''Procedure''' | '''Procedure''' | ||
| + | |||
see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol4</a></html> | ||
| Line 20: | Line 23: | ||
'''Materials''' | '''Materials''' | ||
| + | |||
*QB Buffer 1800μl | *QB Buffer 1800μl | ||
*PE Buffer 4200μl | *PE Buffer 4200μl | ||
*EB 300μl | *EB 300μl | ||
| + | (sum volume) | ||
'''Procedure''' | '''Procedure''' | ||
| + | |||
<html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html> | <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol5</a></html> | ||
Revision as of 21:04, 23 October 2010
E.coli Fiber Project Notebook
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2010/09/07 Tuesday (Bambi75)
Agarose Gels Electro-phoresis
member
bambi75 and watachin
Materials
- 10×Loading Buffer 30μl
- DNA maker 15μl
Procedure
see protocol4
collect DNA
member Same above
Materials
- QB Buffer 1800μl
- PE Buffer 4200μl
- EB 300μl
(sum volume)
Procedure
