Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/24

From 2010.igem.org

(Difference between revisions)
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==2010/08/24 Tuesday(bambi75)==
==2010/08/24 Tuesday(bambi75)==
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===Experiment:Agarose Gels Electrophoresis===
===Experiment:Agarose Gels Electrophoresis===
'''member'''
'''member'''
 +
NEX , bambi75 and watachin
NEX , bambi75 and watachin
'''Materials,Procedure'''
'''Materials,Procedure'''
 +
Same protocol on August 16th.
Same protocol on August 16th.
PCR Product of insert are bcsA and bcsC.
PCR Product of insert are bcsA and bcsC.
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'''Procedure'''
'''Procedure'''
 +
Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
Please see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80PCR">protocol3</a></html>
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==Experiment:Transformation==
+
===Experiment:Transformation===
'''member'''
'''member'''

Revision as of 18:41, 22 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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Contents

2010/08/24 Tuesday(bambi75)

Experiment:Agarose Gels Electrophoresis

member

NEX , bambi75 and watachin

Materials,Procedure

Same protocol on August 16th. PCR Product of insert are bcsA and bcsC.

Experiment:PCR

member

NEX , bambi75 and watachin

material

  • sterilized water 213μl
  • Ex taq buffer 30μl
  • dNTP 24μl
  • Ex taq 3μl
  • K12bcsA sense 15μl
  • K12bcsA antisense 15μl

Procedure

Please see protocol3


Experiment:Restriction enzyme digestion

member

Same above

Materials

  • PCR production
  • m buffer 30μl
  • BSA 30μl
  • Xba1 6μl
  • Spe1 6μl

Procedure

Please see protocol4


Experiment:Transformation

member

Same above

Materials , Procedure

Please refer to protocol9