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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/24
From 2010.igem.org
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| - | ==Experiment:Agarose Gels Electrophoresis== | + | ===Experiment:Agarose Gels Electrophoresis=== |
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PCR Product of insert are bcsA and bcsC. | PCR Product of insert are bcsA and bcsC. | ||
| - | ==Experiment:PCR== | + | ===Experiment:PCR=== |
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*BSA 30μl | *BSA 30μl | ||
*Xba1 6μl | *Xba1 6μl | ||
| - | * | + | *Spe1 6μl |
'''Procedure''' | '''Procedure''' | ||
Revision as of 18:39, 22 October 2010
E.coli Fiber Project Notebook
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Contents |
2010/08/24 Tuesday(bambi75)
Experiment:Agarose Gels Electrophoresis
member NEX , bambi75 and watachin
Materials,Procedure Same protocol on August 16th. PCR Product of insert are bcsA and bcsC.
Experiment:PCR
member
NEX , bambi75 and watachin
material
- sterilized water 213μl
- Ex taq buffer 30μl
- dNTP 24μl
- Ex taq 3μl
- K12bcsA sense 15μl
- K12bcsA antisense 15μl
Procedure Please see protocol3
Experiment:Restriction enzyme digestion
member
Same above
Materials
- PCR production
- m buffer 30μl
- BSA 30μl
- Xba1 6μl
- Spe1 6μl
Procedure
Please see protocol4
Experiment:Transformation
member
Same above
Materials , Procedure
Please refer to protocol9
