"
Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/23
From 2010.igem.org
(Difference between revisions)
| Line 14: | Line 14: | ||
*EX taq polymerase 1µl | *EX taq polymerase 1µl | ||
*milli-Q 71µl | *milli-Q 71µl | ||
| - | |||
'''Procedure''' | '''Procedure''' | ||
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol3</a></html> | follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol3</a></html> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
===Experiment:Restriction enzyme digestion=== | ===Experiment:Restriction enzyme digestion=== | ||
'''Member'''<br /> | '''Member'''<br /> | ||
Bambi75 and watachin | Bambi75 and watachin | ||
Revision as of 18:29, 22 October 2010
E.coli Fiber Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2010/8/23 Monday (watachin)
Experiment:Direct PCR(K12-bcsA,K12-bcsC)
Member
Bambi75 and watachin
Materials
- E.coli K12 colony
- 10µM/l forward Primer 5µl(K12-bcsA,K12-bcsC)
- 10µM/l reverse Primer 5µl(K12-bcsA,K12-bcsC)
- 10×EX taq buffer 10µl
- 2.5mM each dNTP mixture 10µl
- EX taq polymerase 1µl
- milli-Q 71µl
Procedure
follow the protocol3
Experiment:Restriction enzyme digestion
Member
